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KMID : 0545120190290030382
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Journal of Microbiology and Biotechnology
2019 Volume.29 No. 3 p.382 ~ p.391
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Discarded Egg Yolk as an Alternate Source of Poly(3-Hydroxybutyrate-co-3-hydroxyhexanoate)
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Hong Yun-Gi
Moon Yu-Mi
Hong Ju-Won
Choi Tae-Rim
Jung Hye-Rim
Yang Soo-Yeon
Jang Dae-Won
Park Ye-Rim
Brigham Christopher J.
Kim Jae-Seok
Lee Yoo-Kyung
Yang Yung-Hun
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Abstract
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Many poultry eggs are discarded worldwide because of infection (i.e., avian flu) or presence of high levels of pesticides. The possibility of adopting egg yolk as a source material to produce polyhydroxyalkanoate (PHA) biopolymer was examined in this study. Cupriavidus necator Re2133/pCB81 was used for the production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) or poly(3HHx), a polymer that would normally require long-chain fatty acids as carbon feedstocks for the incorporation of 3HHx monomers. The optimal medium contained 5% egg yolk oil and ammonium nitrate as a nitrogen source, with a carbon/nitrogen (C/N) ratio of 20. Time course monitoring using the optimized medium was conducted for 5 days. Biomass production was 13.1 g/l, with 43.7% co-polymer content. Comparison with other studies using plant oils and the current study using egg yolk oil revealed similar polymer yields. Thus, discarded egg yolks could be a potential source of PHA.
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KEYWORD
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Egg yolk oil, Cupriavidus necator, medium optimization, feed stock, co-polymer
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